Glycoprotein and biotin-coupled proteins as taxonomic markers of Cassia fistula [Glikoproteinai ir su biotinu poromis sujungti baltymai kaip taksonominiai Cassia fistula žymekliai]. – Botanica Lithuanica, 11(3): 135–140
Protein, glycoprotein and biotin-coupled protein band patterns of three Cassia species were studied. The leaves of C. fistula L., C. occidentalis L., and C. siamea Britt. showed different protein band patterns after extracting, resolving by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and staining with Coomassie brilliant blue solution. No common bands were observed among the three species. Based on glycoprotein and biotin-coupled protein detection, the 53 kDa bands were clearly seen as markers for the studied species, and 43 and 33 kDa bands appeared as those of C. fistula. The band markers appeared repeatedly without any influence from pollution stress. The leaf sampling method by submerging petioles under water for 3 days also did not show any effect on these band markers. The 53, 43, and 33 kDa glycoprotein and biotin-coupled protein bands can be used for the identification of C. fistula.
Keywords: biotin-coupled proteins, Cassia fistula, glycoprotein, taxonomic marker.