Detection of natural infection of tomato crop by Potato X potexvirus [Aptikta bulvių X poteksviruso natūrali infekcija pomidoruose]. – Botanica Lithuanica, 13(3): 203–209
Samples of diseased tomato (Lycopersicon esculentum Mill.) plants of cultivars ‘Luseta’ and ‘Au�riai’ were found in Kaunas and Vilnius districts. Naturally infected tomato plants had symptoms suggestive of virus infection. Symptoms of infection were exhibited by general interveinal-yellowing of leaves, bright mottling, reduced leaf size and slight stunted plant growth. These tomato samples were analysed using transmission electron microscopy (EM), test plants and reverse transcriptase-polymerase chain reaction (RT-PCR) technique. The identification of virus was based on results of determined experimental host range, symptomatology, morphology of virus particles (about 500 nm in lenght) and specific cDNA amplification fragments size (360 bp) of virus RNA in RT-PCR. Data on the virus purification and use of this preparation for virus detection by PCR are presented. The obtained results indicated that the virus isolated from tomato plants had properties characteristic to Potato X potexvirus (PXV). This is the first report of tomato as a natural host of PXV in Lithuania.
Keywords: tomato, Potato X virus, detection, RT-PCR.